Why is SDS-PAGE incorporated into Western Blot over native PAGE?

I know both can be used, but why is SDS-PAGE "preferred"? Does it have to do with cross-reactivity?

29 March 2020 6,000 5 View

Is there an easy way to determine the area of a raster inside a polygon/shapefile?

I have a (1) polygon/shapefile of administrative boundaries and a (2) a raster with different categories (e.g. 1, 2, 3, 4, 5,) I would like to determine the area of the raster (only category 1)...

18 October 2018 4,545 5 View

Did someone are using RNAstable with RNA samples in a elution buffer instead of DPCE -traeted water?

31 December 2017 2,045 1 View

How to get representative sample of fungi/micellia bacteria from a reactor 10L?

Hello, I am dealing with the sampling of fermentations which becomes very viscous due to micellia. We have a sampler but I suspect the tube is too thin and we are not getting a representative...

30 August 2017 2,174 3 View

What's the usual molarity for acid wash? (Nitric acid to wash away metals in glassware)?

I'll be making use of zinc solutions and as much as possible I don't want to cross-contaminate my samples

12 April 2016 5,548 1 View

What would be the surface morphology change of Moringa oleifera seed powder (biosorbent) in these photos using a Scanning Electron Microscope?

My study involves protonating (through nitric acid) Moringa oleifera seed powder (lignocellulosic) to, theoretically, increase adsorption capacity.In literature, acidifying causes oxygen surface...

26 March 2016 2,008 3 View

Does somebody have a good experience by teaching some programming course by using a pseudolanguage like Karel or Logo?

One hypothesis is that by doing that, students can learn programming faster...

04 May 2014 1,520 1 View

Does anyone know any siRNA transfection reagent for primary cortical neurons, besides Lipofectamine?

I'm using lipofectamine now but would like to try any other reagent.

03 November 2013 1,529 6 View

Which book would you recommend for a first course in "Pattern Recognition" ?

I would like to have comments not only from teachers but also from students, as their opinion is quite important.

11 May 2013 4,789 17 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View