Insect Lipase Activity?

Hi everyone! My name is Nacho. I am trying to assay lipase activity from insect midgut supernatant, and I have some problems and some doubts about it. I do not have the pH-stat. Thus, the...

22 November 2020 5,595 3 View

Is Tetrabutylammonium hydroxide Mass Spectrometry compatible?

Hello forum, I would like to know if TBA.OH is volatile in mixtures of acetonitrile or methanol. I want to analyze peptides that were previously dissolved in TBA.OH-conatining solution, but dried...

05 October 2020 6,936 3 View

Any good manual for phenomenological approach to sustainability?

I'm thinking about a turn from my ethnographic approach to a phenomenological approach in the study of sustainability and forest preservation. However, I didn't find any good manual to design and...

06 July 2020 9,618 1 View

Can RL agent's decision be explained after inference?

I would like to know if there is a method, or sets of method that can explain why a RL agent has chosen certain decision, after the agent has been trained. I'm not looking for different RL...

23 April 2020 151 3 View

Can you desalt peptide digests with high pH RPLC?

Hello! I am working with some materials that require desalting of the peptide samples prior testing. My samples contain a salt of potassium. With a standard sample, I would use R3 or C18 resins...

19 January 2020 6,772 3 View

Why is the TMB substrate turning blue without being in contact with the sample and in the dark?

Hi everyone, last time I was doing an ELISA without exposing it to the light and before I added the substrate it was turning blue. Do you have any idea or suggestion of a better substrate?, the...

03 January 2020 1,294 7 View

Hello. Does XPowder has capability to carry out Rietveld refinment? Where can I find it in the software?

Hello XRD experts. I am getting acquainted with analyzing XRD data using XPowder. Does anyone know whether XPowder has the capability to carry out Rietveld refinement? If so, where do I find in...

02 January 2020 1,162 2 View

Methods available to calculate animal density by means of camera trapping?

We have two large camera trap data sets (tropical and temperate forest), which I would like to use for estimating animal density. Which are the available methods? Strengths and weaknesses? Thanks

16 January 2019 5,879 3 View

Where can I buy olfactometers for entomological use?

Good morning, I need to know where I can buy olfactometers, whether in y shape or four ways for entomological use, the idea is that in the odor chamber small samples can be disposed (essential...

11 January 2019 2,227 4 View

I have to remove RNA from DNA samples. Using a RNAase A (4 mg/ml), which volumen do you recommend to use?

I have some sample that I have to re-purify and some samples to isolate DNA de novo. In both cases I have to use RNAase to remove RNA. I tried with 1.5 ul of RNAase A (4 mg/ml) but the RNA is...

13 December 2018 860 2 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

01 March 2021 3,905 5 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View