I have never been working on lung cancer, since my experience is on HCC and breast cancer, but in the seminars I have attend I have seen people using two different ways:

1) Intranasal administration 

2) intravenous administration

Hope I can help you to run your experiments.

Alvaro

Thanks Alvero..

But if i use the NNK for lung cancer development, then which method would be more faster for development of lung cancer in rats.

Nisat

If you want to use the NNK model, the standard method is i.p. injection (2mg mouse in saline).

You can follow:

- Ruo-Kai Lin, The journal of clinical investigation (2010)

- Megan D. Siwicky. Lung Cancer (2011)

Good luck,

Thank u very much.