How Can conduct measurement on powder using Raman Microscopy?

I am trying to measure the Raman microscopy spectrum of a drug in its powder form and I am not sure what is the best way to spread the powder over the microscope slide so that no interference will...

13 December 2020 7,884 6 View

In your opinion, Does Aflatoxin B1 cause a mutation in BARCA1gene and thus produce triple negative breast cancer?

In your opinion, Does Aflatoxin B1 cause a mutation in BARCA1 gene and thus produce triple negative breast cancer?

15 May 2020 608 52 View

What does ImmunoGrid mean and is it important in developing immunotherapy, vaccines and drugs?

What does ImmunoGrid mean and is it important in developing immunotherapy, vaccines and drugs?

08 April 2020 177 60 View

How to calculate pH value of concrete ?

I have a concrete specimens and I would like to find the pH value for the concrete at different depth of the specimen?

30 January 2019 1,848 4 View

Data formatting issues in HIERFSTAT?

I'm trying an analysis on HIERFSTAT for the first time using a subset of my data. I have some formatting issue that I can not put my finger on because when attempting to run basic.stats and other...

05 December 2018 9,820 2 View

Bacterial adhesion and pH effect - can anyone help?

Can anyone explain why some bacteria's adherence and biofilm formation is more enhanced in alkaline medium than acidic medium?

30 September 2013 2,271 3 View

Antibiofilm activity assay

I worked on antibiofilm activity of some enzymes by crystal violet assay on 96- wells , but what's the best way to destaining CV 95% ethanol or 33% glacial acetic acid and why ?

19 December 2012 6,257 6 View

Teichoic acid of staphylococcus spp.

Does anybody know the molecular weight of Teichoic acid from staphylococcus spp.?

05 July 2012 9,509 1 View

Klebsiella EPS matrix

Hello everybody , is the EPS matrix of Gram -ve biofilm same in chemical composition ? or has anyone has an article that reveling the chemical composition of the EPS matrix of klebsiella biofilm...

03 January 2012 655 1 View

Bactericidal effect to Gram Negative Bacteria

Hello to everyone , i am working on klebsiella , so, has any one list of enzymes that can kill klebsiella or gram negative in general . pleeeeeeeeeeeeeees .

01 January 2012 1,163 1 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

How to show a protein is being dimerized in SDS-PAGE gel?

A protein dimerizes through CTD. I have expressed CTD of protein with two different tags. Now, I want to show that these CTD of protein dimerize in a pull-down assay. But my doubt is that if the...

29 January 2021 7,529 4 View

Fuzzy regulator applied to the asynchronous machine?

hello, in fact I encountered problems during the simulation of the fuzzy regulator applied to the asynchronous machine. I replaced the PI regulator of the vector control with the fuzzy regulator,...

12 January 2021 6,131 3 View

What type of plants is better to work in gene regulation control project ?

I Interested in to work in gene network modeling thus I need to know what is future of gene network modeling in agriculture thanks

04 January 2021 6,527 3 View

Most appropriate fit of a standard curve in an enzym-linked lectin assay?

Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...

03 January 2021 1,010 6 View

Global change in protein expression?

Dear all, When I run my yeast total protein lysate extracted after 10 minute-treatment with my compound, I observe a global up regulation in proteins, even my loading control protein. I suspect...

03 January 2021 1,388 8 View

No title

It is important for me to know the opinion from your point of view as researchers.

30 December 2020 988 2 View

How do I know if my E.coli cells are fully lysed from sonication ?

Hi everyone, I am doing protein expression via E.coli. I am required to lyse the e.coli cells via sonication to obtain cell lysate. I normally sonicate 1L cell pellet suspended in salt buffer at...

22 December 2020 9,675 7 View

Problem with GST-fusion protein elution- Can anyone help?

18 December 2020 4,559 6 View