In besides of physico-chemical properties have found two recent studies such as Natural environment and laboratory environment both are different. But taking in note chemical compound like ammonia, nitrite, nitrate and conductivity and temperature have been performed for normality. focusing on temperature: In natural the temperature could be 12.3 - 38.6 Celsius and in lab 26-29 Celsius. So I hope between this temperature values you may find quality of production with physico- chemical property test.

Hello, Hasan, if I understand your points correctly, here is my answer.

You have three treatments (3 temperatures regime) with triplicate tanks for each treatment. And you have 18 fish per tank or treatment (It's not clear). Whatever you have, your design fits for ANOVA. You can employ Shapiro-Wilk's test and Levene's test to check data normality and homoscedasticity, respectively before ANOVA. I hope it will help you. Best wishes.

Hello, Jaikul Islam. I do agree with your standard point of the tests. I was trying to say with precision temperature variations in eggs (other components which related to lab fish tanks)and results from recent studies For data count. Without standard tests the results will not be approved.