Hello all,
I was hoping to get your advice on what type of test I should be completing for my current research.
As a quick explanation of my research I am currently looking at a certain cell type within the brain, using both treatment (WT vs mutant mouse) and sex as variables. For this I was running a simple two way anova to determine differences within my dependent variables.
Where I was looking for help is recently I have completed a second developmental time point study, and would like to compare the findings between my embryonic time point to the postnatal one to see if the differences within the dependent variables are significantly relevant. Normally I would think to do a repeated measures ANOVA, but these two groups are not of the same subjects, so it throws a bit of a wrench.
Papers similar to the work I'm completing, who also would be using different samples from each development point, mention using a repeated measures all the same. Just as they are, I am working within a mouse model system between my two time points that are raised under the same conditions.
Considering this information, would it be best to run a 3 way ANOVA where I have time, sex, and treatment as factors? Or should I look to follow similar literature which used a repeated measures two way ANOVA despite the subjects being different.
Please let me know if anything needs further clarification to help with your understanding of my question.
Thank you,
Sarah
Hello Sarah,
It sounds to me as if the 3-way anova makes the most sense (assuming that WT and treatment mean exactly the same thing in both batches), as the batches are independent. Maybe there is some fancy tip-toeing that others have tried to portray such data sets as longitudinal/repeated measures, but I don't see it.
Note that the age dimension is not randomized, but more like a blocking factor (as for sex of the animal).
Good luck with your work!
Hi Sarah!
I think you should use the Mixed ANOVA Test, as your experiment design is between- and within- subjects at the same time.
I hope I answered your question.
Best.
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