Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

Do you know anyone who might be interested in running oncology related randomized studies concerning vit. D as profilaxex for oncological problems??

There have been a number of studies relating vitamin D deficiency to cancer and relapse in different types of cancers, for instance Hodgkin's lymphoma. Yet it does not have any repercussion for...

09 November 2020 9,211 2 View

What is the most accepted probabilistic criterion considering frequency layers (fire) via distribution modeling?

fire index prediction

15 September 2019 3,112 2 View

Could you please suggest some research works about psycology of language?

Dear Sir/Madam, At this moment I'm currently working in my dissertation project. The question is "in which way is the teacher of portuguese as a foreign language conditioned by his culture in...

24 December 2018 5,861 2 View

Cultural awareness in medicine - who in the UK runs a course for medical students?

Dear colleagues, Together with my colleagues at the Medical University of Warsaw we are developing a short course on cultural awareness and multiculturalism in medicine. The course is primarily...

09 May 2018 8,306 5 View

Minimal number of generators of a k-algebra over commutative rings

08 August 2017 8,849 3 View

I am trying to calibrate Samsung Galaxy S7 to get real colour from a piece of paper in VR.

I am trying to calibrate Samsung Galaxy S7 to get real colour from a piece of paper in VR. Any recommendation?

14 May 2017 6,388 1 View

MIC concentration and bacterial concentration?

So when using MIC we are basing our numbers established on a bacterial concentration. For instance we say the mic of citric acid for salmonella is 1000ppm. We did the test at a starting bacterial...

09 May 2017 1,870 9 View

The administration of the high fat diet 60% for 10 weeks is efficient in inducing obesity and insulin resistance in C57BL/6J mice?

high-fat 60% for 10 weeks C57BL/6J mice Obesity and insulin resistance

03 May 2017 9,711 5 View

I plaque a 24-well plaq. with C6/36 cells, but after 24h the cells didn't grow up. What can i do to work?

C6/36 cells, Arbovirus. Cell culture. Virology.

23 April 2017 7,869 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View