How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

Where can I buy BrHPP (bromohydrin pyrophosphate) for cell cultures????

I need to get BrHPP for expanding Tgd cells, so if anyone knows about a biotec/chemistry company that has it commercially available I would appreciate the information. Thanks!

20 December 2020 7,022 3 View

Is Uniform Convergence property contradictory to No Free Lunch Theorem?

In Statistical Learning Theory, Uniform convergence means for a hypothesis class H, given m(\delta, \epsilon), for arbitrary distribution over domain (X, Y), with probability $1- \delta$, the...

03 November 2020 7,732 5 View

HX temp1 out as a function of flow rate1 in?

Trying to derive a transfer function for a cross flow tube and fin HX (liquid to air, tube is air side & hot side). Temperature control feedback loop, temp sensor for feedback and valve for...

23 August 2020 1,738 3 View

What is the meaning of Positive delta G In Free Energy calculation between protein and Ligand?

I have done Gromacs for FEP ( Free energy perturbation) between Protein and Ligand in Water solvent, with Positive Ions. I got DG 26.02 +/- 3.14 Kj/Mol. I got positive Delta G. How i can...

18 August 2020 736 4 View

Time in While Loop ?

Hello , I have a problem . I want to calculate the time in m / s from While loop so that I can integrate the values. I tried a lot, unfortunately that didn't work. I use the MPU6050 accelerometer...

03 August 2020 4,518 3 View

How to calculate RECEIVER OPERATING CHARACTERISTICS(ROC) curves and area under the curve (AUC) of microRNA data?

Hello, I am having micro RNA data sets of control and patients now I am interested into analyzing micro RNA as a biomarker for a particular disease. I have already delta delta CT values for...

30 June 2020 7,858 2 View

Does colour difference delta E, always increases with increase in drying-air temperature?

I dried tomato samples in a hot-air dryer, varying the air temperature at 3 levels, 45, 55 and 65oC. Result obtained showed that samples dried at 45oC had the lowest colour difference as expected...

21 June 2020 3,449 2 View

Heaviside funtion on delta(phils) function in COMSOL?

Hello everyone. I want to apply the Heaviside function on the delta(phils) as shown in the figure. Can I apply the step function as a Heaviside function shown in the figure?

19 May 2020 310 7 View

What is the qPCR value that i should use for p value test?

My sample size is 18 including patient and control . I have used target gene and reference gene . Many replays are recommended the delta ct value, but still unclear why i should use it ? what...

27 March 2020 723 4 View