Which Protease inhibitor i should use with the lysis buffer when extracting protein from the brain?

30 July 2022 0 2K Report

I always use the protease inhibitors cocktail and RIPA to extract the whole protein from brain tissue. But i found that the cocktail including the protease inhibitor of metalloprotease，since my protein of interest is MMPs, i'm afraid it will have effect on the results of band as the inhibitor will interact with MMPs forming protein complex. Is it a problem i should care?

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