Something is wrong with my Raman spectra of TiO2, Carbon dot and CD TiO2 composite. What can be the reason?

I have already taken XRD and FTIR for these samples which are fine. Pls check attachment.

20 September 2020 7,351 7 View

How to use both right and left axes of a graph in HFSS?

I want to plot both efficiency and gain in the same graph. How can make right side vertical axis as a gain and left side vertical axis as an efficiency (%) or vice versa?

22 July 2020 1,502 3 View

I am planning to express my protein in Pichia and would like recommendations on the best expression kits or individual components for expression?

I have no prior experience with expression in Pichia. I would like recommendations on individual Pichia cells and vectors, or expression kits. I also want to make decisions about secreted /...

19 July 2020 1,308 1 View

How to test MIMO antenna on vector network analyzer(VNA)?

The designed antenna is 2X1 MIMO antenna. I want to test its return loss on VNA. How to apply input to MIMO antenna? Shall I test one antenna at one time or both antennas should be excited at the...

24 September 2019 7,702 8 View

Is pET21 vector compatible with Rosetta host cells?

pET21 contains ampicillin gene as the selectable marker and Rosetta cells require Cloramphenicol because of the pRARE plasmid? What antibiotic should I use then?

16 May 2019 4,264 3 View

What could be the possible reason for MCF-7 and HeK 293 to grow in suspension but not adhering to flask?

12 October 2016 1,145 3 View

Why Sample in SDS-PAGE and MALDI TOF show different molecular weight ?

My protein shows single band at 28Kda in SDS-PAGE but for MALDI it shows a single peak at 23 Kda what could possibly be the reason

08 June 2016 999 4 View

How does one determine therapeutic potential of a nuclear hormone receptor?

When you are investigating a particular NHR signaling pathway for the worm development, how do you know whether it has therapeutic potential or not? Is the treatment likely toxic or not? Can you...

10 July 2014 3,040 1 View

Why do I get an amplification curve in negative samples without template? Can anyone list methods to detect contamination of kit or RNA or reagents?

I am using RNA extracted from whole adult female Brugia malayi worms for qPCR studies. I have been using iTaq Universal SYBR green RT kit for my qPCR. From the first run I am getting a separate...

22 May 2014 4,701 3 View

How can I do dissociation of antigen bound to antibody on Protein G sepharose column?

I want to dissociate virus bound to antibody onto the Protein G sepharose high performance columns. Can anyone suggest a simple protocol which will enable me to separate them while keeping them...

19 May 2014 5,788 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

The procedure compute?

02 March 2021 5,204 3 View

Does anyone know what a '.' means in SPSS?

Hi everyone! I was wondering if anyone would be so kind as to help me with regards to my data set on SPSS! I was checking for multivariate outliers via the standardised residuals ('Linear...

02 March 2021 7,910 3 View