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Tuhin Khan

Time resolved fluorescence is always a better technique when differentiating between static and dynamic quenching. Temperature dependent experiment (when dealing with steady state fluorescence intensity) will give some information regarding the quenching mechanism. If you have to choose between time resolved and temperature dependent florescence time resolved is always better. Lifetime is less prone to error and easy to measure. On the other hand temperature dependent study may not be (that too steady state intensity) that easy (small temperature range, solvent evaporation, fluctuating temperature, error in measuring the temperature in the cuvette).

Please go through Lakowicz's Principles of Fluorescence Spectroscopy. Chapter 8. Quenching of Fluorescence

Farah Ansari

Thank you.