Hi folks. So, in my work I'm trying to evaluate if some microsatellites markers are polymorphic, before proceeding with them in capillary electrophoresis. I was thinking of using the MetaPhor™ agarose gel but I couldn't find it for sale in my country (Brazil). Do you know of any other alternative high-resolution agarose gel to evaluate polymorphisms of microsatellite markers? My markers are'll between 200-350bp, dinucleotide.
Dear Victor, thank you for your interesting technical question. Please have a look at the following potentially useful link which might be helpful finding an alternative method:
High Resolution Melting Analysis Is a More Sensitive and Effective Alternative to Gel-Based Platforms in Analysis of SSR – An Example in Citrus
Article High Resolution Melting Analysis Is a More Sensitive and Eff...
I hope it helps. Good luck woth your work!
Hi. I hope the following article could help you very much;
https://www.biorxiv.org/content/10.1101/649772v2.full
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