I have a sub fraction after MPLC then column sephadex LH-20, containing two major compounds, can I go for Recycle HPLC, any suggestion?
Dear Sir. Concerning your issue about the method is better for isolation of compounds from plant extract. I think the following below links may help you in your analysis:
https://www.researchgate.net/publication/287063527_Sephadex_LH-20_column_chromatography_of_the_hydrolysed_lignan_macromolecule_of_flaxseed
https://onlinelibrary.wiley.com/doi/pdf/10.1002/jssc.201700704
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3286800/pdf/nihms-296149.pdf
Thanks
Mr. Shah,
Of course, the method of choice is the chromatography.
Bojidarka B. Ivanova , but which one recycle PHPLC or ?
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