Column choromatography required more solvent and takes more time for isolation, so suggest some methods which can be used for isolation of active constituents from plant extracts, instead for column chromatography?
I immediately think of SPE (solid phase extraction) as an alternative. However choice of SPE column shall strongly depend on sample property. Macherey & Nagel propose to this a nice test sample kit (free of charge) of SPE sample catridges (e.g C18, phenlyl solid phases) easy to handle.
HPLC is not an isolation procedure; but rather a detection procedure.
You may opt for the VLC ( Vacuum Liquid Chromatography)https://www.google.co.tz/search?q=vacuum+liquid+chromatography+procedure&biw=1366&bih=635&source=lnms&tbm=isch&sa=X&ei=DGLPVK2iMsOM7AbH9oDQCg&ved=0CAYQ_AUoAQ#imgdii=_&imgrc=WBa3vYkm7NqLVM%253A%3BsMeeam3aguXAkM%3Bhttp%253A%252F%252Fgerwick.ucsd.edu%252Fresearch%252Fvlc.jpg%3Bhttp%253A%252F%252Fwww.keywordpicture.com%252Fkeyword%252Fvacuum%252520liquid%252520chromatography%252F%3B300%3B225 With increasing polarity in your solvent system, remember to start with less polar (petroleum ether) followed by % of less polar to middle polar (DCM,EtoAC)up to middle polar itself and% of middle polar with polar and ultimately 100% polar ( EtOH,MeOH) .After Concentration if there is a crystals in some of your VLC-Fractions, perform crystallization and if not you may opt for the PTLC(Preparative Thin Layer Chromatography) https://www.google.co.tz/search?q=vacuum+liquid+chromatography+procedure&biw=1366&bih=635&source=lnms&tbm=isch&sa=X&ei=DGLPVK2iMsOM7AbH9oDQCg&ved=0CAYQ_AUoAQ#tbm=isch&q=preparative+thin+layer+chromatography&imgdii=_&imgrc=o_Wuoa5_yVhrcM%253A%3BhMr8_338I_AnOM%3Bhttp%253A%252F%252Flipidlibrary.aocs.org%252FSilver%252Ftlc1%252FFigure04.jpg%3Bhttp%253A%252F%252Flipidlibrary.aocs.org%252FSilver%252Ftlc1%252Findex.htm%3B300%3B225. The combinations of these two methods makes life easier in the research lab and save time, remember to start with enough amount."you are welcomed for more elaborations"
Preparative thin layer chromatography is good only that it requires smaller sample material than Column chromatography. However, you must make right choice of solvent system (eluent).
check conditions for bulk extract absorption on ionic exchange resins. If the product is charged and stable at a suitable buffered pH, the rate of purification will be very favorable and with a bit of good luck you will need only desalting and a preparative HPLC to complete purification.
if the problem is interaction of your sample with solid support such as silica, please try counter current chromatography HSCCC and then GPC - HPLC
Dear Vinesh
A similar question "Can anyone give me the different "recent" extraction methods for medicinal plants?" has been asked by Danaldi Chiroma Husaini. You may be interested in checking the response from other researchers.
Good luck.
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