I am screening some essential oils such as Manuka, Myrtus, Melissa, Eugenol, TTO ecc.. at concentrations ranging from 0% to 8%. I am using Tween 80 to enhance the solubilization of the oil in the media. I read that the normal concentrations of tween 80 used are around 0.5% (v/v) but even increasing the concentration at 4% (v/v) I am not able to obtain a well mixed suspension.

I add the tween 80 to the media, stirring for 1 hours and after I add the oil. 

Am I doing anything wrong? Do you have any idea on why I am not able to mix the oil using the concentrations found in literature?  Should I use other surfactants?

Thank you very much for your help.

Best wishes, 

Elisa Serra

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