I need to purify total RNA from plants (Arabidopsis thaliana and N. benthamiana) for RT-qPCR. I usually use RNeasy Plant Minikit (Quiagen) which work quiet well, but is very expensive. Recently, I heard from a colleague the possibility to use an alternative kit from Epicenter (MasterPure™ Plant RNA Purification Kit). Does anybody have any advice concerning this choice?. Or recommendation for other cheaper methods?
RNeasy Plant Minikit (Quiagen) is cheap, robust and works fabulous ! Best wishes. Biswa
Call up the company and ask for some free samples for you to try before you make a decision to buy.
I isolated total RNA from rice plant using Trizol reagent according to the manufacturer (Ambion) protocol. Isolated RNA had sufficient purity for RT-qPCR.
Regards
The former should be better .I always use it .The RNA isolated from the former has goog quantity and quality.
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