Which is the best Primer for amplifying the 16S rDNA of a Bacteria Isolate?

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Mert Sudagidan

Dear Lukman, I suggest you two paper we use for 16S rDNA sequencing.

Best regards.

Thiago Bruce

I agree with Yemendzhiev. That pair is one of the most extensively use and generate an almost complete 16S gene. But if you are working with tags the V4 and V2 reagions are a good option as well. In this case we can find the easily find the primers using in NGS studies.

Lukman Afolabi

Thank you for your input Thiago Bruce