Which is the best option after DNA amplification with ARTIC V3 protocol to prepare library for SARS sequencing? NebNext or Illumina DNA lib prep kit?

15 April 2021 0 10K Report

I would like to know the best protocol for sars cov2 sequencing in MiSeq

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Can ROBINS-I be used to measure risk of bias in single-arm studies?

I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...

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What are these aggregates in activated sludge flocs?

I've been working with activated sludge samples and have encountered a challenge in identifying certain structures/organisms that appear abundantly within the flocs during optical microscopy...

23 June 2024 9,584 1 View

Extraction of intracellular protein with TRI reagent for ELISA?

I use ELISAs for quantification of extracellular proteins in cell-conditioned media. I am interested in quantifying uncoupling protein 1 (UCP1) which is an intracellular, mitochondrial...

27 May 2024 8,025 1 View

Can Sensory marketing affects customers satisfaction in Hotels?

12 May 2024 3,308 3 View

Is there a simple formula to estimate the prevalence of a disease (in this case, carriage) in a longitudinal one-stage cluster sampling?

Hello everyone, I would greatly appreciate it if someone could assist me in understanding the steps required to estimate the sample size for a longitudinal study. Or advise bibliography. I am...

04 April 2024 2,882 0 View

Should I block compensation beads with mouse serum in flow cytometry?

I am using mouse serum to block Fc receptors before staining, for flow cytometry. My question is if I should also add the serum to compensation beads before staining them, so that the cells and...

07 March 2024 9,989 2 View

Can staining for flow cytometry be done in flat bottom 96-wells plates?

Most staining protocols for flow cytometry in 96-well plates use V-shape or U-shape plates. I would like to ask if staining could also be done in flat-bottom plates. Thank you!

07 January 2024 2,910 5 View

Do FMO controls for flow cytometry need to be run in each experiment?

I plan to run my experimental samples in the flow cytometer in batches. My question is if FMO controls have to be prepared and run every time I run experimental samples, or if I could only do them...

04 January 2024 7,842 0 View

Statistical analysis for PBMCs immunophenotyping by flow cytometry?

I am immunophenotyping PBMCs by flow cytometry using markers for T cells to analyse the different subsets (Naive, CM, EM and EMRA), and to measure senescent and exhausted t cells. I want to...

02 January 2024 5,387 1 View

Which plasmid to use for CRISPR in Lewis Lung Carcinoma and subsequent injection?

Hi, we've been unsuccessful in creating single clones with deletion of a couple of proteins, after testing several sgRNA sequences for each. We are using a lentiviral vector encoding puromycin for...

25 December 2023 2,901 2 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

How to fix install package meta in R?

> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’

18 July 2024 3,536 0 View

Analysis of MHC-I and II alleles with CNVs and unassigned loci?

I am working on a dataset of MHC-I and II alleles from a bird species sequenced with Illumina. We were not able to assign alleles to loci through MHC-typer as we were over the limit of 150 alleles...

15 July 2024 182 1 View

Language teachers can develop speaking skill through listening practices. Can you please suggest some more ways to promote the same (Speaking Skill)?

"Today several adjectival phrases have been used to describe English like ‘International Language’, ‘Lingua-Franca’, ‘Language for Globally Connecting’, ‘Library Language’, ‘Official Language’,...

01 July 2024 8,406 5 View

Are there different databases similar to the Zinc database or how can I solve this problem in the Zinc database?

We were using the Zinc database for the virtual compound library in our studies, but there have been problems downloading a large number of compounds for a while. Are there different databases...

30 June 2024 8,144 1 View

Can I directly ligate Illumina adaptor to the end of ssDNA?

I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...

26 June 2024 4,522 0 View

Who can recommend where to buy Flow Cells for Illumina HiSeq 2500?

I recently acquired a used Illumina HiSeq 2500 and am in need of flow cells compatible with this machine. As I am relatively new to this aspect, I would greatly appreciate any recommendations on...

22 June 2024 5,992 1 View

Do we have any python package for implementing contexual bandits (used in Recommendation Systems) ?

How can contexual bandits (used in Recommendation Systems) be implemented in code via library package ?

18 June 2024 3,196 6 View

How can I purify filamentous fungi from leaf tissue?

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12 June 2024 4,027 1 View