Which is the best method to collect CSF samples from rat brain, from cicterna magna and without opening the skin in this area?

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Mitra Sadat Shirazi

you can find that in attached paper

Athineos Philippu

Sorry, no experience. We are interested in dynamics and continuous CSF collection that can be achieved using the push-pull superfusion technique. Wilhelm Feldberg has donne a lot of work concerning your question..All the best Athineos Philippu

Malleswari Challagundla

Yes it is possible to collect the CSF by puncturing with needle attached to a butterfly collecting tube (similar set up generally used for blood collections in humans) at cisterna magna without opening. It is more or less like a lumbar puncture in humans, But you really need to practice to get the feeling where to puncture exactly (gap between the neck and spine) without bleeding. you can collect from 25 to 90µl per time, but can varies based on the animal age and size.

Sedat Develi

You can check these articles

https://www.ncbi.nlm.nih.gov/pubmed/19109998

https://www.ncbi.nlm.nih.gov/pubmed/20005255