Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

Which sampling techniques can be used for huge flying insects?

Tropidacris collaris is a grasshopper. Adults, which can reach more than 15 of length, are strong flyers and actually they resemble small birds or bats. Most of the time T. collaris is found in...

13 January 2021 3,735 5 View

Does freezing treated cell pellet at -80C damages them?

Hello, I hope you are all doing well. I have a question regarding my treated cells group. I plan to do western blot and qRT-PCR for my treated cells. However, to save time, I would like to...

06 December 2020 1,390 5 View

Why do some primers have transcript variants/unintended templates for different genes?

Good day, I hope you are all doing well. I was designing primers for Caspase 9 and sometimes, the designed primers in NCBI have transcript variants for the same gene (Caspase 9) but at the same...

05 October 2020 352 3 View

Do I need to design forward and reverse primers within an exon region for QRT-PCR?

Good day, I hope you are all well. I am new in designing primers for quantitative reverse transcription PCR. I am using NCBI to design my primers and my question is: Do I have to design my...

04 October 2020 7,678 7 View

Why is my molecular rotor displaying a bathochromic shift in toluene compared to MeOH or EtOH?

Hello, I am studying the photophysical properties of a molecular rotor which unexcpectedly, shows a lambda(max, absorption) = 565 nm in toluene, but a lambda (max, absorption) = 540 nm in MeOH...

15 September 2020 7,830 3 View

Animals - Special Issue, "Learning Theory Applied to the Welfare of Animals", looking to submit?

Dear Colleagues, Please see the following link and information for more details. If you are thinking about submitting, please feel free to reach out to me directly here or at...

09 September 2020 6,361 1 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

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02 March 2021 3,060 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

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28 February 2021 9,936 2 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

What are the important topics in the field of Artificial Intelligence in Business world?

Dear All, I am doing my MS in Electronic Businesses. And I am interested in the AI field and its applications for businesses, its benefits, impacts, etc. What kind of scientific research dominate...

26 February 2021 8,816 6 View