Hello Hyacint Mtz

Is it the problem with isolation of Phytophthora or for getting the inoculum? it is to promot the sporulation of Phytophthora by taking  about one cm away from the edge of  fungal colony and put it in petri dish containing distilled water plus leaf discs of potato or tomato, after 24 h you can get good sporulation and sporangium as well. Pease see the attached references:

Good luck

Hi Hyacint

This reference is guide for identification "Practical guide of Phytophthora"

https://research.cip.cgiar.org/confluence/download/attachments/37192003/Drenth_Phytophthora_Practical_guide9.pdf?version=1&modificationDate=1273703622000

and "Improved methods for use in studies on Phytophtohora cinnamomi"

https://www.tandfonline.com/doi/pdf/10.1080/00288233.1965.10420024

And "Phytophthora"

https://www.phytophthoradb.org/pdf/2008APS_kelly.pdf

And "Phytophthora2"

http://agrifoodscience.com/index.php/TURJAF/article/viewFile/325/219

This also "An efficient baiting assay for quantification of Phytophthora cinnamomi"

http://onlinelibrary.wiley.com/doi/10.1046/j.1365-3059.2000.00478.x/pdf

Also "Phytophthora3"

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=0ahUKEwi06pq1mODRAhWBCJoKHV43Cv8QFghXMAg&url=https%3A%2F%2Fwww.apsnet.org%2Fpublications%2Fphytopathology%2Fbackissues%2FDocuments%2F1995Articles%2FPhyto85n10_1057.pdf&usg=AFQjCNF_0mDPNH6gVkoXnEzC0Vy1CQzwXQ&sig2=k2LawVyabm8V-9c2zQsdSA

and "Phytophthora4"

http://phytosphere.com/soilphytophthora/Phytophthora_and_nurseries.htm

And "Identification of Phytophthora species baited and isolated from forest"

https://grunwaldlab.cgrb.oregonstate.edu/sites/default/files/efp12015.pdf

Also"Collection of Samples to Test for Phytophthora"

https://www.dpaw.wa.gov.au/images/documents/conservation-management/pests-diseases/dieback/phytophthora_sampling_guidelines_v3_11-5-2007.pdf

And "Using green pears to bait for Phytophthora"

https://phytosphere.com/soilphytophthora/pearbaitingPhytophthora.htm

And "An efficient method for zoospore production, infection and real-time"

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377013/pdf/12870_2015_Article_470.pdf

And "A quantitative Real Time PCR based method for the detection"

https://www.sciencedirect.com/science/article/pii/S1319562X13000892

And "BAITING FOR PHYTOPHTHORA AND PYTHIUM IN PRODUCTION"

https://horticulture.com.au/wp-content/uploads/2016/11/10-Nursery-Paper-Oct-2016.pdf

Also "Rapid Diagnostic Tools for Phytophthora on Horticultural"

https://usablight.org/sites/default/files/upload/image/Workshop%20lab%20protocols_0.pdf

Also "Methods for Isolating Phytophthora"

http://horticulture.ucdavis.edu/main/Deliverables/Ristaino_phytophthora_isolation_methods.pdf

Also "Phytophthora5"

http://aciar.gov.au/files/node/598/mn114-part3.pdf

And "Cultural characteristics of Phytophthora fungi"

https://www.savzv.sk/domain/b6/files/foc/foe_32_no2/foe_vol32_no2.pdf

also "Comparison of Two Media Selective for Phytophthora and Pythium"

https://www.apsnet.org/publications/PlantDisease/BackIssues/Documents/1986Articles/PlantDisease70n11_1038.pdf

And 'Identifying Phytophthora species"

https://fhm.fs.fed.us/sp/sod/misc/culturing_species_phytophthora.pdf

And "Selective Isolation of Species of Phytophthora from Natural"

http://www.nature.com/nature/journal/v223/n5206/abs/223636a0.html

And 'Evaluation of selective media and bait methods"

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.1986.tb02055.x/abstract?systemMessage=WOL+Usage+report+download+page+will+be+unavailable+on+Friday+27th+January+2017+at+23%3A00+GMT%2F+18%3A00+EST%2F+07%3A00+SGT+%28Saturday+28th+Jan+for+SGT%29++for+up+to+2+hours+due+to+essential+server+maintenance.+Apologies+for+the+inconvenience.

Also "Phytophthora phighters"

http://www.crssa.rutgers.edu/projects/gps/peter_proj/PARP%20and%20isolation.doc

Thank you very much.!!!

Dear Hyacint  Mtz

,I think that the best method to isolate Phytophtora from soil is to use apple fruit trap. In order to get that you have to chose apple fruit, wash it carfully, then sterilize it in suitable solution, wash it again in sterilized distilled water then immersed it until its half.  Irrigate the soil and leave the fruit till appearance white mycelium in area of the fruit between soil and air, then you can isolate   on suitable medium.

Thank you, the Apple method is great indeed.