Which filter is used to get the wavelength of 570 nm and 660 nm, what is the logic behind selecting filter for any wavelength in a microplate reader?

More Prakash Kishore Hazam's questions See All

Which one among gram positive and gram negative bacteria posses greater membrane negative charge and why. Any citations?

Teichoic acid and Lipopolysaccharide creates negative charge over bacterial membrane in Gram positive and Gram negative bacteria.

04 May 2016 3,602 0 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

s. Béatrice Marianne Ewalds-Kvist

Blue Optical Kit

Ex: 490 nm

Em: 510 - 570 nm

Red Optical Kit

Ex: 625 nm

Em: 660 - 720 nm

Read: http://fi.promega.com/products/pm/fluorometers-luminometers-multimode-readers/glomax-multi-plus-instinct/

Rasbindu V. Mehta

Optical filters are characterized by their wavelength and bandwidth. Depending upon type of the reader bandwidh may be selcted. For example for flourence interference filter may be preferable since it has a narrow bandwidth.

Takashi Kuroda

If you look for a filter with tunable wavelength, an option might be http://www.semrock.com/FilterDetails.aspx?id=TBP01-628/14-25x36 - you need to tilt it for wavelength tuning.

Yes-tilting filter can have tunable wavelength-but range is limited.

Prakash Kishore Hazam

Thankyou everyone for your kind and resourceful reply