Which database can be considered for creating cytoscape network?

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Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

Why wait for a doctor's visit when you can become the guardian of your child's health today?

Join our exclusive WhatsApp group and access the latest research-based videos on children's health! Discover the hidden toxins and other risks that may harm your kids, and learn the best practices...

04 August 2024 1,264 0 View

When you express a protein, why do we express not only the domain we want, but also the protein around it?

I want to express STK4, and I've searched the paper for reference. When I check the protein kinase domain sequence for that kinase on Uniprot, it's 30-281, but the paper expresses the protein...

20 July 2024 4,951 1 View

Is it okay to use reviewed proteins?

I'm working on the proteome (from Uniprot), as you're probably aware that there are reviewed and unreviewed proteins, however unreviewed proteins are in large quantities, but reviewed proteins are...

09 July 2024 5,782 2 View

Protein Sequence Similarity or Sequence Identity?

Hi fellow researchers, I have got a noob query regarding the question of the post. I am trying to build a data set of enzymes (from UniProt data base) that would contain protein sequences and...

21 April 2024 7,281 4 View

Which is the suitable protein-protein interaction data analysis method/platform for identification of targets from MS/MS?

I have a list of identifiers/hits processed based on log fold changes. I tried using pantherDB to generate GO terms and cytoscape for network construction. I need a clear picture on how to...

02 January 2024 8,770 2 View

Tool for annotation of protein list?

Hey there, I have a list of proteins (up to 300) listed by their gene names. I would like to see their subcellular location (based on e.g. the uniprot database). Is there a tool where I can enter...

18 December 2023 6,116 4 View

How to change node-edge binding position in Cytoscape Desktop?

Dear colleagues, I am trying to plot a protein-protein interaction network in Cytoscape. My idea is to place the node representing the bait protein on one side of the figure, and on the other...

18 December 2023 9,182 2 View

Why is maxquant not recognizing uniprot database fasta file?

We also tried to identify peptides from the yeasts used during the fermentation process and for such we tried to apply the Saccharomyces sp. and other Saccharomyces protein databases from UniProt....

09 November 2023 5,863 1 View

Why good sleep is of great importance to people and how to achieve it?

Good sleep is necessary for people and is just as important as proper nutrition, physical activity or oral hygiene. Sleep problems not only make daily survival difficult, but are also associated...

04 October 2023 4,494 1 View

Stepwise protocol on constructing target-pathway network using cytoscape. Particularly how to prepare pathway excel sheet?

Network Pharmacology

25 September 2023 6,202 1 View

How do I construct a target pathway interaction network on Cytoscape?

I need a stepwise protocol to construct target pathway interaction network on Cytoscape. How do I prepare the excel sheet?

10 September 2023 9,512 3 View