This is really depending on tissue type and species. Some recent good overviews you can find in

Wang, MR., Lambardi, M., Engelmann, F. et al. Advances in cryopreservation of in vitro-derived propagules: technologies and explant sources. Plant Cell Tiss Organ Cult (2020). https://doi.org/10.1007/s11240-020-01770-0

Panis B (2019) Sixty years of plant cryopreservation: from freezing hardy mulberry twigs to establishing reference crop collections for future generations. Acta Hortic 1234:1–8

But for organized tissues like meristem cultures, the PVS2 droplet vitrification method could be a good start

Cheers

Bart Panis, PhD

Senior Scientist │ Genetic Resources

Representative of the Alliance of Bioversity International and CIAT in Belgium

Alliance of Bioversity International and CIAT

Europe – Belgium, Leuven

c/o KU Leuven │ Willem Decroylaan 42 │ P.O. Box 2455 │ 3000 Leuven, Belgium

✉ [email protected] │ 📞 +32 (0) 16 321690 │ Skype bart.panis

Dear Sabuj Das,

The preferred cryopreservation method or combination of methods will vary with the crop and is dependent upon the equipment available, technical skills, budget, and project goals. Therefore, there is no best or standard cryopreservation method. The selection of a protocol depends on a range of criteria, but often begins with a review of the literature to identify previous successes in similar or related crops.

The papers that Dr. Bart Panis recommended are great and with recent advances in cryopreservation.

I am also sharing a recent paper on Challenges in implementing plant shoot tip cryopreservation technologies

https://doi.org/10.1007/s11240-020-01846-x

Best regards,

Jean

Thank you Dr. Bart Panis and Dr. Jean Carlos Bettoni for your kind and informative answers. It'll really help me onwards.

Droplet vitrification has been very promising for our avocado cryopreservation work.