23 July 2014 4 4K Report

Commonly, Glycoside hydrolases (also called glycosidases or glycosyl hydrolases) assist in the hydrolysis of glycosidic bonds in complex sugars.

More Dr. Sushil Nagar's questions See All
Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

What is the best method for preparation of Polymeric nanoparticles for co-delivery?

We are planning to make Polymeric nanoparticles for co-delivery of 2 flavanoides. Which is the best method, that one should follow.

09 February 2021 7,086 8 View

OmniScriptum Publishing Group?

Hello, Has anybody published with OmniScriptum Publishing Group. I got an email from them. The emails claims the editor is from the group but the email is signed out by lambert academic...

08 February 2021 5,611 3 View

Following is XRD of iron oxide nano material synthesised using bipyridine derivatives. What are the reasons for Broad Humps?

Even after few repetitions..I am getting similar results. Is is due to organic components?

20 January 2021 9,279 6 View

How much Ti and B (in ppm) is required for effective grain refinement and why?

To have the desired grain size in 6061 and 6063 Al alloy, what should be the minimum % of Ti and B pick up and what is the chemistry/metallurgy behind that?

07 January 2021 9,305 2 View

How to make AlV50% alloy by thermit route?

Kindly provide the technical papers/patents on the production of AlV50% alloy by thermit route, by using V2O5

05 January 2021 2,471 3 View

Apart from deoxidation what are the other applications of Ferro Aluminum (FeAl) and Al wire?

FeAl and Al wire has applications in Steel for deoxidation. I want to know the other application of various grades of FeAl. Does Al wire also has other applications apart from deoxidation? Regards

27 December 2020 8,435 1 View

Is there is any reference if someone is using Ti sponge cored wire instead of FeTi 70% cored wire for plain carbon steel?

There is a different behavior in carbon steel and stainless steel. Drastic low recovery in carbon steel due to high melting temp. But in ss , working temp is high so dissolve at 1668 degree temp....

16 December 2020 8,765 8 View

I need Coronary Angiogram Images for Coronary Artery Disease detection?

I need Coronary Angiogram Images(CAI) publically available dataset. Coronary Artery Disease (CAD) is a condition of the heart due to atherosclerosis. Atherosclerosis is the narrowing of arteries...

07 December 2020 6,470 3 View

Similar questions and discussions
ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

I have proposal to extract HPA from snails, dos any researcher have protocol of HPA (Helix pomata aglutinin) extraction?

Lectin fro Helix pomatia agglutinin used to detect O-Linked glycosylation

21 October 2020 6,139 3 View

How do I Glycosylate a Protein?

I am in my first year of a biology degree and i'm struggling with some coursework. How would i go about setting up an expression system to create a glycoprotein. I understand how to over express a...

19 March 2020 6,605 4 View

What are the specific enzymes which plays pivotal role in iso-peptide bond formation like Transglutaminases?

Enzymes from the lyase, ligase, and hydrolase classes have the ability to catalyze carbon-carbon bond formation. Likewise, I want to know about specific enzymes (like Transglutaminases) or class...

20 February 2020 5,862 3 View

Can I study three partner interactions with Surface Plasmon Resonance?

We are looking at whether a peptide: RNA as a complex can bind to a glycosylated protein. We have established with SPR that the Peptide binds to RNA. Is it possible to study the binding of the...

01 February 2020 5,807 2 View

Quantifying glycosylated proteins on a SDS-PAGE?

What is the best way to approach (unexpected) glycosylated proteins on a western blot (or in general)? I am looking at some potential anti-fibrotic treatments (Tx) using 2D cultured primary human...

01 February 2020 7,303 3 View

What are the specific enzymes which plays pivotal role in iso-peptide bond formation like Transglutaminases?

Enzymes from the lyase, ligase, and hydrolase classes have the ability to catalyze carbon-carbon bond formation. Likewise, I want to know about specific enzymes (like Transglutaminases) or class...

01 February 2020 2,143 2 View

Is there any difference between p-glycoprotein and MDR1 protein?

Hi, Can anybody tell me is there any difference between these two proteins? I am doing western blotting for expression analysis of MDR1 protein. So I am using MDR1 monoclonal antibodies. Predicted...

10 November 2019 1,939 7 View

Does anyone have a protocol to purify glycosylated fibronectin from serum samples?

I am trying to purify glycosylated fibronectin from serum samples. Unfortunately, there is no specific antibody against glycosylated fibronectin. So, I want to use lectin-based affinity...

11 September 2019 1,426 3 View

What are the essential cofactors to perform in vitro assay using alpha/beta hydrolase enzyme ?

Need to perform in vitro reaction with substrates and purified protein (Methyl esterase/alpha/beta hydrolase super family) 1. What are the essential cofactors? 2. Which buffer solution/pH should I...

14 April 2019 9,298 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close