Grain boundary misorientation close to 60 degrees: is that necessarily a twin boundary?

Steel grain boundary misorientation close to 60 degrees: is that necessarily a twin boundary? Can it be just a high-angle grain boundary?

31 December 2017 3,562 6 View

Heat-treatment for 316L austenitic steel in order to reduce/remove the defects after plastic deformation without recrystallization

Which heat-treatment should be applied to 316L austenitic stainless steel in order to reduce or remove the defects after plastic deformation without recrystallization?

01 February 2017 5,596 6 View

How to increase the grain size in 316L steel?

Which heat treatment should be applied in order to increase the grain size in solution-annealed 316L steel?

10 November 2016 5,650 5 View

Which process leads to higher work-hardening rate of austenitic stainless steel: cross slip or planar slip?

Which process leads to higher work-hardening rate of austenitic stainless steel: cross slip or planar slip?

07 August 2016 3,187 12 View

What is the exact reason of the occurrence of the banded structure in austenitic stainless steel?

03 April 2016 266 11 View

Which technique is more reliable for quantification of the phases in steel: EBSD or XRD?

I need to define the amount of ferrite in austenitic stainless steel. Which technique is better: EBSD or XRD?

10 November 2015 755 57 View

Do the hardness measurements require any specific surface preparation of the samples?

I am planning to measure the hardness of my steel samples. Do I need to do any specific surface preparation (grinding, polishing, etc.)?

10 November 2015 4,819 6 View

Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

Nuances in the digital twin concept. Opinions?

Hello everyone, I am currently working on the state of the art of the digital twin concept, and after seeing the categorization made by Kritzinger, W. et al. (2018) [Digital Twin in...

14 February 2021 2,107 3 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Xrd result of ZnO thin film shown better crystallinity at low temperature and the crystallinity decreases as the temperature increased. why?

I have synthesized ZnO thin films at four different substrate temperature say, 300, 350, 400 and 450C. and the samples were annealed at 450C for half an hour. the xrd result shown better...

04 February 2021 7,641 4 View

How can I partially remove the metal oxide layer for solar cell device?

Hi, I want to make a perovskite-based planar solar cell device. (electrode/HTL/perovskite/ETL/FTO) To fabricate the device, one electrode should be deposited on HTL and the other should be...

01 February 2021 229 6 View

Are there any technical and safety tips or details for hot salt baths?

29 January 2021 6,036 2 View

Do you have experience of second round PCR amplification issue?

When 1st-round PCR amplification was finished , I could get a clear expected band, but the amount was very low, so I cut the band and run second-round PCR with same condition, it didn't work, I...

21 January 2021 888 4 View

Sb2Te3 thin film annealing and damage(break)?

21 January 2021 9,642 2 View

Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View