I want to extract total alkaloid from plants. I have tried several methods and still not get the alkaloid. The method that I used is:
1. 500 gram sample is boiled with water for 30 minutes and add lime juice 50 mL (pH 3), then squeezed (3X). Results of the filter boiled again until the solution becomes viscous. Once cooled, add caustic soda to pH 11. The solution was fractionated using petroleum and petroleum fractions evaporated.
2. 100 gram samples soxhleted using methanol. The extract was concentrated fractionated using n-hexane to remove chlorophyll and fat. The remaining methanol extract was added 50 mL of acetic acid and homogenized for 30 minutes. The extract was then added with 50 mL of 10% caustic soda and fractionated with ethyl acetate. Ethyl acetate fraction evaporated.
Whether the method used is correct? or is there any other method is better?
Best regards,
Ayu Muthia
Presence of alkaloid was tested qualitatively by Dragondroff's method then subjected to quantitative estimation by UV-Spectrophotometer. This method is based on the reaction between alkaloid and bromocresol green (BCG). Probably this can help.
Agree to Ashutosh
Please refer attached method for estimation of Total Alkaloids
You can follow this procedure:
For the purpose of phytochemical analysis of the selected plants,
0.2 g of the selected plant samples were added in each test tube and
3 ml of hexane were mixed in it, shaken well and filtered. Then took
5 ml of 2% HCl and poured in a test tube having the mixture of plant
extract and hexane. Heated the test tube having the mixture, filtered it
and poured few drops of picric acid in a mixture. Formation of yellow
color precipitate indicates the presence of alkaloids
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Pharmaceutical Botany
- Plant Extracts