Kindly tell me types of neurones present and their surface receptors?
In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...
19 June 2024 7,822 3 View
Dear colleges, I'm a dental graduate (BDS) and did my master's degree in public health (MPH) with two years of experience. I'm working as a Scientist-B in one of the Indian Council of Medical...
02 July 2023 1,959 0 View
Hi, To understand participants' life satisfaction, 5 questions have been asked. In each question, there are 5 options, from disagree to agree. Please suggest me how to create a single variable...
01 April 2022 7,014 5 View
Recently, I have studied the impact of land surface temperature (LST) on an important soil erosion factor the cover management factor (C factor). My study was on the winter season. It shows a...
15 January 2022 4,397 3 View
During setting up assembly cloning reaction, we need to add the insert and vector in ratio 1:4, which is mentioned in the protocol. But I don't know what is the reasons behind this ratio. I will...
28 June 2021 419 2 View
For my Doctoral thesis, I am framing a quantitative questionnaire tool to collect healthcare seeking behavior of vulnerable tribal peoples. The content of questionnaire were synthesized from...
23 June 2021 8,853 4 View
I have generated so many models having a different coefficient of determination (r2) and RMSE. In this analysis, it has been seen that it is not mandatory that a model having maximum r2 has a...
15 July 2020 6,239 4 View
In many situations in practice, the explanatory variables (xi) may not remain independent due to various reasons. The situation where the explanatory variables are highly intercorrelated is...
07 July 2020 6,689 21 View
I am using ALOS-2 data. After radiometric terrain correction, I have got Gamma naught image. After that, I just want to have the Sigma naught image from that Gamma naught image.
03 May 2020 8,617 0 View
I have stuck with the interferometry of Sentinel-1 data in the phase of coregistration. The SNAP gives java heap space error several times for two weeks. I am finding any freely available software...
20 April 2020 1,295 5 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
Can
08 July 2024 6,725 4 View
Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...
03 July 2024 3,884 5 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...
30 June 2024 6,502 5 View
I'm trying to use this novel material (LaZrO2) as a gate oxide in SOI using 'user.material' syntax but getting an error
28 June 2024 3,150 1 View
When choosing the receptor model (rigid or flexible) in molecular docking, does it depend on the designed ligands' characteristics or anything else? For instance: + With the ligand A, the protein...
25 June 2024 2,716 0 View
