Which is the most economical and effective method?
Dear Gabriel,
1. In lipid extracts from animals, cholesterol is generally the only sterol to be determined. If the amount of cholesterol present in a convenient aliquot is estimated to be in the range 1-10 µg, a colorimetric method can be run.
2. If the cholesterol amount is too low to be estimated by colorimetry (range 0.01-1 µg in the aliquot) or if other sterols are present (food or plant extracts) or if all sterol compounds must be quantified, two techniques can be used:
Quantification by HPLC
Quantification by GLC
For the details of the analytical methods mentioned above and other important information, please use the following link:
http://www.cyberlipid.org/sterolt/ster0001.htm
Hoping this will be helpful,
Rafik
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