Hi,

I'm doing a test to assess the hemolysis of some plasma samples and I have seen that one of the best methods is to see the absorbance at 414nm using Nanodrop (reference: Article Comparison of Methodologies to Detect Low Levels of Hemolysi...

I tried using the Nanodrop 2000 and the Nanodrop One, but the results are really different, for example, in the first sample in Nanodrop 2000 I obtain a value of 0.075AU and in the Nanodrop One a value of 1.75AU, and this is the same for all of the samples.

Someone knows the difference between how the Absorbance Units are calculated in both cases?

Thanks in advance,

Lluc