Is there multiple-alignment tool which would not neccassarily align all sequences together?

When I get results from sequencing, they often overlap only partly or not at all. In such case it's easier to align them with the "parental" sequence (of what I expect), to see where they align....

01 February 2020 8,336 3 View

A simple method for quantitative separation of apoplast and plant cells?

Hello everybody, I wish to separate apoplast from the rest of the plant (i.e. intact cells) to see whether the compound I treat the plants with goes inside or stays outside. When I mentioned this...

07 August 2019 2,886 3 View

How to make a dot plot?

How do I make a dot plot like in this paper Show the data, don't conceal them in Figure 1? All I'm finding on the Internet are only this type of dot plots:...

05 June 2019 2,092 5 View

How can I improve binding to chitin beads?

I express my protein in pTYB12 vector as fusion with intein and then try to purify on chitin beads. Typically, I load overnight at low flowrates (something like 0.3 ml/min). The loading buffer is...

04 May 2019 2,589 7 View

How to determine mode of action of a certain compound?

I'm working with plant hormones cytokinins - derivatives of adenine with a side chain at the N6-group. They are mostly active as free bases (with no modification on the adenine core), or maximally...

02 March 2019 3,322 11 View

I have two questions regarding adenine conjugates at N9 position /?

My first question concerns lupinic acid - conjugate of adenine derivative with alanine https://commons.wikimedia.org/wiki/File:Lupinic_acid.svg The question: What type of bond is it between the...

08 September 2018 8,949 0 View

Is the cell lysis efficiency by ultrasound affected by cell density and/or the volume?

Hi everybody, I'm currently expressing proteins in and extracting from bacteria. The procedure is that I pellet the bacteria from bioreactor, resuspend in buffer (50 mM Tris/HCl, pH 8.0; 1M NaCl;...

07 August 2018 2,453 9 View

I have a few questions about growing E. coli in bioreactor - and the obligatory question mark?

Hello everybody, I'm currently growing E. coli in bioreactor and I have experience only with P. pastoris in bioreactor, so I'm not sure, how should it behave. With Pichia, one can try hard to...

02 March 2018 9,364 4 View

Does ligand have affinity towards protein or vice versa?

In the texts, it's often written, that the ligand has affinity towards a protein (with appropriate affinity constant). However, to me it always occurred that the protein binds the ligand and...

09 October 2017 6,994 0 View

How common is substitution at position 7 of adenine ring?

Some old papers (from the 70s) claim, that the substitution at position 7 is rare in the nature https://www.ncbi.nlm.nih.gov/pubmed/486500https://www.ncbi.nlm.nih.gov/pubmed/4640371 However, that...

09 October 2017 7,784 2 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Add text at the beginning of a specific reference in Latex?

Hey researchers! The journal I'm submitting to wants datasets to be listed in the reference as [dataset] Authors; Year; Dataset title; Data repository ; Version; Persistent identifier (e.g....

18 November 2020 2,681 5 View

How do you include the literature in reference list that is not cited in the text?

Hi, as I know you should not cite the authors if you already include them in the sentence. But if I do not cite the study then it will not show in the reference list (I am using Zotero). How do I...

25 October 2020 9,203 3 View

Can anyone provide assistance with creating a pilot study to validate a survey I created?

I've created a survey to use in my dissertation study. It asks teachers to rank ten factors (1-10) based on their influence on the participants' job satisfaction. The factors included came from...

20 October 2020 1,373 3 View

How to invite call centers to participate in your study?

I'm conducting a job satisfaction study to predict telephone agents' turnover. I'm looking to increase participants for my study. How should I approach the directors of call centers to request...

20 September 2020 7,099 8 View

Should I use multiple regression analysis to proceed with my quantitative data analysis?

I am doing a research project at a hospital, and the goal is to investigate the relationship between job satisfaction and turnover intention. So far, I have identified the groups of employees...

15 July 2020 2,176 13 View

No title

09 July 2020 9,914 1 View

How to avoid incomplete responses?

I'm conducting an anonymous quantitative survey on SurveyMonkey and some participants left the majority of the questions unanswered. How can we encourage participants to completely answer all...

22 June 2020 8,202 4 View

What is the key of Job Satisfaction?

Job satisfaction is relative from one person to another. What do you think is the key to job satisfaction ??

25 May 2020 844 82 View