16 August 2024 1 2K Report

We are on Long Island, and are shopping for agarose cheaply. Just something to make gels with, nothing extremely pure needed.

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Acid Rain and Spruce Bark Beetles now Climate Change, Monocultures, and desicated soil, what changed?

So were we wrong about acid rain in the 1980s? Can we be sure that the new story: Climate Change, Desiccated Soil, and Monocultures, is correct? Another alternative from the 1980s is the massive...

10 July 2024 4,667 7 View

What is the current status of augmented learning in robotic surgery?

I would like to perform a literature review at this time on augmented learning and learning augmented algorithms to enhance performance-guided surgery

06 July 2024 246 1 View

Are we able to upload our publications onto this site?

I have a number of peer reviewed articles that were published that might be useful on this site. I don't know how articles appear on this site, but I would upload mine if acceptable. Thanks.

23 June 2024 2,344 1 View

Help with mounting meningeal tissues with Prolong Diamond?

Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...

05 June 2024 624 2 View

How to extract RNA from powdered plant tissue stored in RNAlater?

My research group has samples of plant tissue that were pulverized in ln2 using a mortar and pestle before being weighed into tubes and stored in RNAlater at -20 degrees for a few months before...

28 May 2024 7,274 0 View

Can we enlist the help of hydrocephalus patients to determine if large scale Bose Einstein condensates occur in the brain?

My research, in particular my analysis of time perception, suggests that consciousness correlates with a large scale quantum coherent state in the brain in the form of a Bose-Einstein condensate....

16 April 2024 2,819 0 View

How much of AI is acceptable and ethical in research?

I ask this question from the perspective that AI algorithms can automate tasks, analyze vast amounts of data, and suggest new research avenues. It can also improve research efficiency and speed up...

15 April 2024 9,158 8 View

What do you think about engineering students using Artificial Intelligence from their first year of studies?

Introducing Artificial Intelligence in the first year of engineering studies offers students a foundational understanding of its principles and applications. This early exposure fosters relevance,...

11 April 2024 3,934 2 View

What are the effects on humans with shortening Childhood; the time between infancy and adolecence?

Humans have the longest childhood of any animal known. There are two huge growth spurts in our lives where cascading hormones shape our bodies. Infancy and adolescence, with the same surges of...

08 April 2024 2,355 22 View

The impact of monetary policy on the lending practices of listed banks?

ANY SCHOLAR WITH A SIMILAR WORK OR HAVE INTEREST IN THE ABOVE TOPIC?

23 February 2024 4,328 3 View

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Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

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Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

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PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

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How to check pH of a high strength hydrogel?

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30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

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23 July 2024 6,664 6 View

Why my gel electrophoresis has smearing?

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How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

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