Where Second Harmonic Generation (SHG) study can be done?

More Priyanka Mule's questions See All

I have got 22-25 colonies on my after transforming the ligation in DH5aplha. Should I screen all the colonies?

I have screened 10 colonies out of 22. But neither get insert release nor the PCR amplification for the desired gene. What should I do, should I further screen the colonies?

02 December 2020 3,225 3 View

Which bacteria can grow on 10x PBS buffer?

I have prepared PBS buffer and components for M9 medium and I store it in freeze, I do not use it if it is more than 2 weeks old. From time to time I check the sterility of these solutions and...

04 November 2020 9,427 6 View

Which model can be used to predict on below data?

I want to predict valuation using verbal SAS but there exists a strong nonlinearity between the two variables which makes unfit to use linear regression. Please see the attached scatter plot image...

05 August 2020 1,602 3 View

Which is the best model to predict student marks based on entrance exam result?

Hi, I have a dataset that contains student data that are nested based on different schools where more that one student belongs to a particular school. student_id school_id non_verbal...

29 July 2020 9,825 18 View

One step RT-PCR enzyme optimisation, can anyone help?

Helping others is the way we help help ourselves! In this case literally!! I work at a biotech company called Genaxxon Bioscience GmbH in Germany. We have an amazing one step RT-PCR enzyme which...

13 July 2020 7,395 2 View

Do Acetonitrile type (gradient grade or non gradient grade) affects the HPLC results?

Trying to develop the HILIC-RID method for sugar analyte but getting very different results between two-run of the same sample. Tried 60 minute washing between two-run. The mobile phase is 75:25...

11 June 2020 8,385 3 View

Why Pseudomonas fluorescens ATCC13525 does not grow on acetate?

I am trying to grow Pseudomonas fluorescens ATCC13525 on different carbon source except glucose in M9 medium but i cannot see them growing on acetate. I am not able to find more information about...

20 February 2020 2,469 6 View

Which funding agency in India will grant traveling allowance for international conference in finance ??

I get an opportunity to present my paper in international conference on June 2020. I am research scholar in management (finance department).Kindly let me know which funding agency will grant...

07 January 2020 7,040 1 View

Is raman peak at 515cm-1 normal for silicon wafer with 532 laser and 600 grating?

We installed new grating to your Renishaw inVia Raman microsscope. While routine calibration of the system, we observed the Si raman peak at 515cm-1 for 532 lase 600 grating and 519.9 cm-1 for 532...

02 December 2019 5,582 3 View

Why does my freshly thawed and seeded HeLa cells look different than usual?

I had stored HeLa cells in -80C freezer for over a month. When I thawed and seeded the cells today, I noticed that the cells looked significantly different than usual cells when floating in media....

25 November 2019 4,352 6 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Complex wave form in ANSYS?

Is there a way to input wave speed in a complex form on ANSYS? The wave speed I need to assign a material is 325(1+0.11i) however I have been unable to do this thus far. Failing that, is there...

27 February 2021 5,969 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

How to generate AA6061 alloy or any aluminium alloy crystal in material studio?

I need to carry out molecular dynamics simulation on Aluminium alloy. For this i need the crystal structure that which could be further replicated to form a structure.

22 February 2021 1,410 1 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

Bikshandarkoil R Srinivasan

For growing your crystal did you use L-tartaric acid or D-tartaric acid or DL-tartaric acid or meso-tartaric acid. It is quite important to specify which isomer is employed for a study rather than just writing tartarate.

Priyanka Mule

Bikshandarkoil R Srinivasan Sir, I used L-tartric acid to grow crystals.