Can conventional HindIII and a FastDigest MssI be mixed together to have double digestion?

Dear Colleagues, I bought a traditional HindIII enzyme and a FastDigest MssI from Thermo Fisher. I wonder if these two can be used together to do a double digestion? If so, what buffer should I...

30 January 2020 6,216 2 View

Anyone has experience on extracting plasmid (9-11KB) DNA on Clonewell 0.8 % E gel?

Dear colleagues, I am in trouble to extract sufficient DNA from the Clonewell E gel. I have an 11 KB plasmid and want to purify it after restriction enzyme digestion to get rid of a 2.3 KB...

30 January 2020 871 2 View

Can conventional HindIII and a FastDigest MssI be mixed together to have double digestion?

Dear Colleagues, I bought a traditional HindIII enzyme and a FastDigest MssI from Thermo Fisher. I wonder if these two can be used together to do a double digestion? If so, what buffer should I...

31 December 2019 2,754 2 View

Will heat shock work to transform large plasmid (15Kb)?

Dear colleagues, I have a question regarding bacteria transformation. I am working with Agro-bacteria GV3101, and want to transfer the plasmid into it. When I get a pure plasmid from cloning,...

26 November 2019 6,598 4 View

Will heat shock work to transform large plasmid (15Kb)?

Dear colleagues, I have a question regarding bacteria transformation. I am working with Agro-bacteria GV3101, and want to transfer the plasmid into it. When I get a pure plasmid from cloning,...

10 November 2019 4,695 4 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

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02 March 2021 932 1 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View