Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How to plot non-linear kinetics model for column sorption study?

Do I have to solver-add inn for its calculation?

10 December 2020 5,209 7 View

How to check the radiation characteristics of IR thermal emitters using COMSOL?

I have designed a MEMS-based IR thermal emitter and done the electro-thermal simulation to get the temperature profile of micro-heater. Now I want to check the radiation intensity vs wavelength...

14 October 2020 4,542 2 View

Why many biochar producers find it difficult to sustain in different parts of the world?

Biochar (solid product with more than 60% carbon as per IBI) has been extensively studied for its production, characterization, and applications. In spite of offering numerous benefits in diverse...

23 April 2020 9,534 1 View

Cloning a 4.3kb insert into 6kb vector?

I really need tips for cloning 5kb inserts into 6kb vector. I am trying the cut n paste method using REs. Which ligation ratio should I use? What precautions should I consider? I would be really...

24 November 2019 9,429 2 View

Please tell me what is better subroutine or python script for simulation of grain and grain boundaries on ABAQUS?

Also if possible please suggest me from where I can learn to write accurate subroutine for microstructure simulation because I am new on subroutine writing.

09 August 2018 6,126 1 View

How can I avoid E. Coli adherence to glass cover slips ?

I am imaging very high concentration of E. coli solutions under confocal microscope. However, the bacterial cells always stick to the cover slip. Is there a coating which prevents their sticking ?...

07 July 2018 6,407 1 View

Hii everyone, my spss paired t test value is coming different when i calcualte manually. i have checked many times. which is correct

my sample size is 43. when i am applying in spss its coming 13.5 and manually 0.88, what should i do

31 May 2017 2,409 5 View

How to model Resin flow for composites in Abaqus/ Msc. Patran?

10 May 2017 9,267 1 View

Can someone help me prove the approximate coefficient of numerical diffusion?

Hi, Can someone please help me with deriving that false/numerical/artificial diffusion is flow direction dependent and it is given as (image attached). also as per Patankar book , numerical...

30 October 2016 6,390 11 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View