Do I need to make a special plasmid for bisulfite methylation test?

I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...

06 January 2021 7,218 2 View

How to perform cell transfection and Dual Glo assay with low-adhesive (HEK293) cells?

Hello everyone. I need to use Promega Dual Glo assay for HEK293 cells, but I am really afraid of wash out my cells from the 96-well plate, and at the same time, I am afraid to use coating because...

09 September 2020 4,817 7 View

How to reduce auto fluorescence in bone-marrow smears that would be used for FISH and IHC?

We are processing clinical samples of bone marrow smears, and have encountered autofluorescence that makes subsequent FISH and IHC virtually impossible. The problems are: 1. the samples are of...

18 December 2019 4,676 4 View

Antibody detects protein which was ablated in null mice (global knockout). Anyone had similar experience?

Here is a dilemma: there is a line of null mice (global knockout), which are null by genotyping and by mRNA analysis (qPCR). They were generated by replacement of an entire protein-coding region...

16 March 2019 9,722 6 View

Does high fidelity taq polymerase get less efficient after expiration date?

24 September 2016 5,468 4 View

Wrong or no fragment insert in directional TOPO cloning?

17 July 2016 10,020 10 View

Why in the analysis of cyanide, ninhydrin dissolved in sodium carbonate?

I was doing research related to the analysis of cyanide with ninhydrin, in some journals mentioned that preparation ninhydrin by dissolving it in sodium carbonate, but I do not understand why. Is...

12 July 2016 8,541 4 View

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15 June 2016 5,924 6 View

Why do I get a wrong length of PCR result but after sequencing it is actually a right target sequence?

Can somebody help me? I am doing a PCR of worm tissue. I used KAPA Taq Extra HotStart ReadyMix. I should be getting a PCR result of 158bp but I always get a 200 bp PCR result and after I sequence...

15 June 2016 7,313 6 View

What are the causes of PCR failure? i change the PCR master mix, optimized the annealing temperature and so on. but still no defined product band?

i want to amplified Channa striata COI gene using universal fish primer, out of 13 samples only two showed defined product bands after several optimization. I change the PCR mix, change the...

06 March 2016 854 6 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

When is the right time to stop training a classification algorithm?

In this example giving 400 samples looks like there is an accuracy of 91.5% while training beyond that point, accuracy is getting decreased. Are 400 samples enough to keep for this example? If...

27 January 2021 5,657 3 View

What new can be added in the field of historical studies to implement more realistic programs?

What new can be added in the field of historical studies to implement more realistic programs in dealing with the events of history and interpreting them with scientific sincerity and competence?

26 January 2021 559 1 View

If The Star Sirius Was Described As Red In Historical Times, Why Is It White Now?

Several classical age writers described Sirius as red or reddish or more red than Mars, along with other red and orange stars that are still the same colors described in the past. Sirius has...

05 January 2021 1,080 2 View

Are there mathematical or statistical processing of satellite imagery or digital elevation data in the detection of historical buried rivers?

In the lands with ancient plain sediments, the courses of rivers change dramatically over time for easy movement and the arrival of rivers to an advanced geomorphic stage. Are there mathematical...

02 January 2021 3,098 3 View

Where can we find historical hourly temperature data for a complete year?

Hello everyone, for our project we need a source for temperature data fulfilling following criteria: 1. we need historical hourly temperature data 2. a whole year has to be covered (ideally a...

08 December 2020 1,724 9 View

In the age of Iconic architecture and global trends, can we still design for people? would iconic architecture enhance identities or destroy them?

In historical cities , would iconic buildings add to the character of the cities or change it ? How would we maintain their character , and yet have expressive new buildings ?

03 December 2020 2,740 18 View

How to learn historical or cultural subjects in the Montessori secondary programs?

I am looking for research that demonstrates practical and theoretical approaches. Thank you from now! #Montessori #Montessori Method

29 November 2020 3,339 6 View

How can I find the best subject between architecture, economy, historical site ?

Hi there, I consider to write my final thesis in master architecture. I am trying to find a good link between economy impact , historical site and high rise buildings which I am interested in, but...

16 November 2020 4,682 16 View

Where can I get historic global/national level hydrometeorological data (preferably gridded data)?

Hi, I would like to know where can I get historic global/national (preferably USA) level time-series data for the following (preferably gridded data)? Flood, Drought, Max temperature, Min...

08 November 2020 9,414 6 View