Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

How should private health-care systems be improved to guarantee everyone's right to health?

COVID-19 pandemic has shown big social inequalities in countries with private health-care systems. Governments must pay greater attention to the right to health, that every human being must have,...

31 May 2020 7,357 23 View

I'm trying to set up a TR-FRET experiment in my lab but I'm having some troubles. Could you give some tips?

I'm working with an his-protein and I'm trying to obtain an IC50 between the protein and the biotinylated substrate and a known competitor. But the experiment is not working. I will add the...

20 April 2020 4,485 3 View

Problems with Google Scholar. Do you know how to fix it?

Dear All I am having some problems with google scholar. Some citations to my papers simply disappeared. I know that the number can be reduced when two different versions of a paper are...

01 July 2018 873 60 View

Is organic chemistry predictable?

It seems like organic chemistry consists of learning a bunch of reactions, but is there any qualitative set of rules for predicting the way a set of organic reagents will react? I know that...

30 May 2017 3,980 3 View

Are there any recognized / respected certificates for R programming or data analysis ?

Thinking about online and MOOC-type certificates for R programming and data analysis, are there any that are recognized and respected by potential graduate schools and employers? I guess if people...

11 March 2017 8,044 3 View

Hi all, what is the best method to determine the onset of bird migration and the end (day arrival), using GPS points?

There is a clear evidence in my data, but I would like to have also a statistical evidence

17 September 2016 7,140 4 View

Has anybody used Cas9 knockin mouse? If so do you know if every single cell express Cas9 constitutively?

Has anybody used Cas9 knockin mouse? If so do you know if every single cell express Cas9 constitutively?

29 September 2015 5,799 3 View

Is there a Cre line specific for the immune system?

22 June 2015 7,815 2 View

Is it possible to conduct AFM electrical measurements (such as EFM and KFM) in liquids?

I was wondering is any of the above techniques can be used for measurements in liquids. Thank you!

19 January 2015 3,178 3 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Database of European Football Players Salaries ?

Hello everyone, I am interested in writing about a dissertation about the wages of the football players of the 5 main leagues in Europe. I wanted to know if someone had an up to date data base...

17 February 2021 8,847 3 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Where to buy optically clear, low viscosity, refractive index 1.5-1.4 Silicone Oil ?

For research purposes, it is required to have optically clear, low viscosity silicone oil with a refractive index of 1.5-1.4. It is going to be used in optoelectronic research. Online purchase is...

01 February 2021 9,160 3 View

Lupasol G100 from BASF -- where to buy?

Hello everyone. I am looking to purchase Lupasol G100 (essentially ~5kDa bPEI) for creation of carbon dots for in planta transient gene expression. Thus far, I have only been able to find a...

20 January 2021 7,683 1 View

Can you please tell suggest me a method to conduct "Meta Analysis" on a particular topic such as "Green purchase Intention"?

Actually, I'm looking forward to having suggestive feedbacks on how to conduct "Meta Analysis" on a particular topic such as "Green purchase Intention"? As I'm in my early academic career, I...

12 January 2021 4,224 5 View

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01 January 2021 5,831 1 View

How to simulate the energy yield of an Organic PV module based on the Temperature effects and the albedo effects?

26 December 2020 4,280 5 View