I want to assess the variability effect of salivary cortisol by immunoassay kits on diagnosis of cushing syndrome and other surrenal related pathologies.
I recommend the use of the Clinical Labotatory Standards Institute (CLSI) guidelines for quantitaive methods http://shop.clsi.org/method-evaluation-documents/ (note: this link includes all MV guidelines, including those where the output is nominal). Westgard's book "Basic method validation" is recommended and cheaper than the CLSI docs. Westgard QC site has a lot of valuable free text www.westgard.com. Attached is a 2 pages PDF with the golden scheme/table for my MV (quantitative tests).
I have measured salivary cortisol by EIA kit; you can check this link
http://www.arborassays.com/documentation/inserts/K003-H.pdf
Thank you so much, if you have more data about other methods, it will be a wonderful gift.
I recommend the use of the Clinical Labotatory Standards Institute (CLSI) guidelines for quantitaive methods http://shop.clsi.org/method-evaluation-documents/ (note: this link includes all MV guidelines, including those where the output is nominal). Westgard's book "Basic method validation" is recommended and cheaper than the CLSI docs. Westgard QC site has a lot of valuable free text www.westgard.com. Attached is a 2 pages PDF with the golden scheme/table for my MV (quantitative tests).
There is a great paper investigating this by Miller et al (2013) "Comparison of salivary cortisol as measured by different immunoassays and tandem mass spectrometry" in Pyschoneuroendocrinology 38: 50-57. A very good article. Salimetrics assays come out very well. If you want a Point of Care test for cortisol check our website (www.iprointeractive.com).
I HAVE learnt this paper, its very interesting. But i research especially that present effect of antibody types prepared during immunization on their selectivity and the consequences on the diagnosis
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