Blerta Stringa

6 Questions 6 Answers 0 Followers

Questions related from Blerta Stringa

Do you have any experience with HS578BST cell line?

Hi everyone, I am trying to do some experiments with HS578BST cell line (normal breast cell line) but I have big problems on growing them. I am using DMEM (with 10% FBS) and EGFR supplied every...

11 November 2018 9,889 4 View

Do you know any useful program to read ChIp seq data?

Hi, I found ChIP seq data from GEO. First time I am working with this format and really don't know which program should I use to read them (I have windows in my PC). Thanks in advance

09 September 2018 4,013 1 View

Ligatio troubleshooting. Does EtoH inhibit the reaction? How can purify my sample?

Hi, I am trying to perform a ligation of PCR products in plasmid digested with BbsI and purified from gel with Qiagen kit. The day after transformation i didn't have any colonies and my doubt is...

04 April 2018 4,583 1 View

Does T4 DNA ligase buffer interfire with Electroporation (nucleofector protocol) buffer?

Hi I am trying to electroporate a phosphorilate PCR product in my cell line. As the amount is very low, I was wondering if I can skip a purification step after phosphorilation. Does the T4 DNA...

10 October 2017 9,330 2 View

Troubleshoot with Western Blot. How to fix it?

Hi, I am trying to detect cMyc with W.B I used iBlot semi dry system and Ponceau was quite good. I blocked with 5% Milk and incubated overnight with primar ab (4°). The day after I washed with...

12 December 2016 6,345 8 View

DNA extracted with DNA extract buffer is ok for ddPCR?

Hi, I am trying to do editing via crispr cas9 in cancer cell lines. Since I am looking for a very rare event (HR), I should do clone dilution and after screening with ddPCR. I was wondering if the...

01 January 1970 4,519 5 View