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Hi everyone, I am trying to do some experiments with HS578BST cell line (normal breast cell line) but I have big problems on growing them. I am using DMEM (with 10% FBS) and EGFR supplied every...
11 November 2018 9,887 4 View
Hi, I found ChIP seq data from GEO. First time I am working with this format and really don't know which program should I use to read them (I have windows in my PC). Thanks in advance
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Hi, I am trying to perform a ligation of PCR products in plasmid digested with BbsI and purified from gel with Qiagen kit. The day after transformation i didn't have any colonies and my doubt is...
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Hi I am trying to electroporate a phosphorilate PCR product in my cell line. As the amount is very low, I was wondering if I can skip a purification step after phosphorilation. Does the T4 DNA...
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Hi, I am trying to detect cMyc with W.B I used iBlot semi dry system and Ponceau was quite good. I blocked with 5% Milk and incubated overnight with primar ab (4°). The day after I washed with...
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Hi, I am trying to do editing via crispr cas9 in cancer cell lines. Since I am looking for a very rare event (HR), I should do clone dilution and after screening with ddPCR. I was wondering if the...
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