Has anyone used a microplate reader to measure MMP with JC-1?

I have several patient-derived cell lines that are slow-growing and material is limited, so instead of flow cytometry I wanted to try measuring MMP using a fluorescent microplate reader on 96-well...

19 June 2016 6,444 1 View

How do large training samples need to be selected in the artificial neural network BP method?

In the artificial neural network BP method, how are large training samples usually selected? 80% of the total set, 50%, or 40% and how to choose? If choosing few, the results are not ideal. How to...

15 December 2013 7,938 9 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Has anyone done Archard Wear Simulation of GFRP Composite Plate in ANSYS with ACT extension or UDF?

I'm looking to do a sliding wear (plate-on-plate) simulation of GFRP composite plates with Archard Wear Model. Any hints or example steps are appreciated.

17 February 2021 1,447 2 View

Which is the best software for the simulation of tribological properties of materials?

I want to simulate the tribological properties of solids, liquids, and fluids, please suggest good software.

02 February 2021 3,947 3 View

ASTM or ISO standard test for duplex coatings?

Dear all, I would like to conduct scratch and linear reciprocating tribo-test on my magensium alloy, which has a plasma electrolytic oxide (PEO) layer of about 20 um and 10 - 20 um of Parylene...

25 January 2021 9,709 2 View

Which Method of Statistical Analysis is best suit for making comparisons?

For the purpose of selecting the best one among the 4 design approaches, experimental video surveillance has taken. The dependent variable is the distance (meter) and the independent variables...

21 January 2021 4,936 7 View

Do we become better with vaccination from covid in 2021?

We hope to recover, and the economy improved by using vaccination .and vaccines is needed. The vaccine will protect you from getting ill and then ending up hospitalized. But it's possible that...

02 January 2021 4,194 4 View

How can we measure the wear resistance and toughness for steel wire?

I want to know different testing methods to investigate the mechanical behavior of steel wire

29 December 2020 610 1 View

In your opinion, what could motivate people to wear masks and do social distancing to stop covid-19?

28 December 2020 5,149 56 View

Gear reduction for back-drivability of a motor?

Hi everyone, Does anyone know up to what reduction ratio of gear does a motor remain back-drivable? I want my robot to have safe interactions with the environment and other humans. Can you give a...

26 December 2020 7,142 3 View

Why isn't UV Laser based PCB manufacturing more mainstream yet?

While feeling the shared pain of costly and slow PCB Manufacturing process. Which also makes the hardware creativity and education much costly and slow. I was trying to figure out how can we...

14 December 2020 1,484 1 View