Sample Size for Quasi-Experimental Quants using one-tailed t-test?

Hi, sorry I'm stumped. I'm going to do a study on the leadership styles (using the MLQ Form 5X) of minority women administrators in medical schools. How will I calculate the sample size? Thank...

30 January 2021 2,865 4 View

Prediction of novel precursor miRNAs in viral genomes?

Dear, I performed a multi-sequence alignment (MSA) with all the complete genome sequences of SARS-related viruses. I then used RNALalifold from the ViennaRNA package, with default parameters, to...

06 December 2020 1,704 3 View

Can I calculate mean and confidence intervals of d-prime values?

Hi everybody. I ran an experiment on memory and calculated the percentage of hits as usually reported in the literature. However, the reviewer asked me to transform my hits into d-prime values. I...

21 October 2020 3,859 1 View

Discrepancy between RNA and protein levels in single-cell seq? CD3 mRNA vs protein?

Hi all, A very quick question. I have sorted human T cells from blood using anti-CD3E, CD4 and CD8a antibodies. We did single cell seq on those sorted cells. Analysing the results, I do not see...

19 September 2020 4,953 3 View

What are the nuclei mean number from freeze tissue nuclei isolation protocol?

Hello, everyone! I'm going to start a project with single-cell ATAC-seq for analysis of open chromatin regions from freeze tissue samples. I'm lost about the nuclei concentration. If I made a...

19 June 2020 7,348 2 View

How do I compute for the Pr(Y=1|X=1) H1 and Pr(Y=1|X1) H0 for a priori estimation of required sample size?

I am trying to estimate the required sample size replicate a study which used a probit regression analysis. The paper reported the probit regression results and the marginal effects but I can't...

17 May 2020 4,826 1 View

Paired-test or unpaired-test?

I have a group of PDXs models derived from the same patient. I want to know if the results between differents mice with the same treatment have significant difference or not. The analysis of 3...

24 March 2020 7,004 4 View

How do you deal with your enironmental chemistry data?

Hi everybody! I am PhD in analytical chemistry and I make part of a multidisciplinary team working on the development of a web based solution focusing mainly on treatment and management of...

12 March 2020 5,597 5 View

488 laser emission crosstalk?

Image shows green fluorescence but no green fluorochrome was used in this specimen (HEK 293 cells), only Hoechst (350 nm absorption). Lasers used: 350 (upper right), 488 (upper left) and 568...

10 March 2020 7,321 3 View

Could I use a BAM file output to make a alignment with others FASTA references?

I am wondering about to use a BAM file output as a input to make a alignment to others FASTA reference genome. I have been reading how to get a FASTA formand from my BAM file but I am not sure if...

23 February 2020 9,672 5 View

Software for flow cytometry-like gating of data sets?

I am currently trying to do image based cytometry from fluorescence microscopy images. From the images I extract data such as cell size and a number of fluorescence intensities. Is there any...

09 February 2021 6,278 1 View

Genus name priority from the same publication (Zoology)?

I came across a recent taxonomic work, where two genera were synonymized. Both genera were published in a paper in 1922 by the original author who revised the family at that time. The original...

19 January 2021 1,920 4 View

How to do variation partition for a distance matrix for microbial ecology?

I have bacterial, fungal, and protist OTU table. I want to know to what extent protist and fungi distribution can explain the bacteria distribution. I only know Mantel test could do a correlation...

11 January 2021 4,164 2 View

Why are the lines that I graphed using the data from my PROCESS macro plug in analysis curved/bent?

Hi all, I have recently conducted a regression analysis using the Hayes PROCESS macro. For those of you who are aware, the output of this macro spits out data points that can be plotted to create...

10 January 2021 3,173 3 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

How to determine how much power an antenna can handle?

How can I know how much power an antenna can handle? I know P = I^2*R, but here I only know R = 50 ohms. how to determine, this antenna can handle this much power? I am working on dipole and helix...

05 January 2021 8,722 13 View

How do i handle Ti and/or Aluminium powder. Since in the instruction on the container is written that CATCHES FIRE SPONTANEOUSLY IF EXPOSED TO AIR ?

i am working on max phase preparation......i am a newly joined PhD scholar. I wanted to make MAX phase of Ti3AlC2 , in most of the literature they are using the metal powder of Ti and Al for the...

23 December 2020 7,122 8 View

Nature will take care: How climate change problem will be fixed by nature without human interference?

Everyone knows, the fast changing climatic condition with altered physical factors due to various anthropologic activities. Humans are unable to handle the issue of climate change due to growing...

21 December 2020 9,283 14 View

How to handle Missing Values for Skipped Questions (Systematic Missing) in R ?

10 December 2020 435 4 View

Is there any reputable journal that can publish a research article in the field of Agriculture without charging the author a handling/publication fee?

Is there any reputable journal that can publish a research article in the field of Agriculture without charging the author a handling/publication fee?

01 December 2020 3,408 16 View