11 December 2022 0 6K Report

I was reading a paper where some expressions related to ODMR were not given in any text, I need a text or any journal referance where ODMR starting from EPR is explained in detail.

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CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Effect of post bake on PPR resist?

HI , i am concerned about any resist hardening effect of Post bake my LOR +PPR coated sample @90C for 5 mins. I wanted to do liftoff in acetone but the metals are hard to peel off. I cant use PG...

18 June 2024 5,741 0 View

What are the different ways to apply the boundary conditions for a compact tension (CT) specimen in FEM modelling.?

Can we restrict the vertical and horizontal displacement in two directions for the lower semicircular in bottom hole of CT specimen and apply incremental displacement in the upper semicircular in...

09 May 2024 5,272 4 View

How to separate out the electronic and ionic conductance in metal halide perovskites?

It is very well known that, owing to the soft lattice, and ionic bonds, metal halide perovskites shows easy movements of different ionic species under external perturbation. Due to this the...

28 March 2024 4,925 2 View

How do we isolate only B cells from Spleen/ lymph nodes for Hybridoma generation?

As we know, lymph nodes have a mixed population of cells, how do we ensure only B cells are getting isolated and subsequently fused with myeloma cells? Thanks in advance!

30 January 2024 3,449 2 View

What are the key considerations and steps involved in the simple centrifugation method for isolating lymphocytes?

What are the key considerations and steps involved in the simple centrifugation method for isolating lymphocytes withou using ready to use lymphocytes seperating medium?

29 January 2024 3,222 0 View

Is it possible to utilize agarose low EEO instead of low melting point agarose for COMET assay?

Is Low melting point agarose and agarose having low EEO the same? Is it possible to utilize agarose low EEO instead of low melting point agarose for COMET assay?

29 January 2024 6,834 2 View

How to determine heat transfer rate across water-air interface in Ansys Fluent or CFD post ?

have simulated a hydraulic jump. There is a difference in water and ambient(air)temperature and as such there is heat transfer across the water-air interface. I have used VOF model and Standard...

17 September 2023 5,277 1 View

Why ExpiCHO cells need 8% CO2?

Why do ExpiCHO cells need 8% CO2 levels in the incubator? Is is because they have high metabolic rate, thereby producing more lactate and needing more CO2 to buffer the media? Would like to know...

26 August 2023 6,707 3 View

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1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

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Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

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Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

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Is it necessary to covary exogenous constructs in a structural model?

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