Where can I find a fetal ultrasound images database ?

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

I seed my cell in Opti-MEM?

I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...

07 July 2024 407 0 View

What are these clumps in my transfected PBMCs?

Hello everyone. I cultured isolated PBMCs in Erythroid Expansion Medium for two weeks and then performed electroporation in my expanded PBMCs in order to reprogram them. The first 24h after the...

22 June 2024 346 4 View

After plating mouse primary neonatal cardiomyocyte we do not observe beating after a few days, any suggestions as to how we can trouble shoot?

We coat the plate in poly-D-lysine (50 ug/mL) followed by fibronectin (50 ug/mL) before plating the cardiomyocytes. After isolating and plating cardiomyocytes from 2-4 day old pups, we fed the...

20 June 2024 393 0 View

Medium replacement interfering with cell culture blot results?

We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....

17 June 2024 1,649 0 View

How is skeletal and respiratory muscle ultrasound being used in the clinical setting, and what are the barriers and facilitators?

Are you a qualified health professional working in the acute adult setting? If yes, then we want to hear from you! Link to survey: https://q.surveys.unimelb.edu.au/jfe/form/SV_aWORJRowoBvxC1U We...

15 June 2024 7,978 1 View

Why are reaction forces so high when I add vibration to cutting model?

Hi I am trying to model a cutting problem with a blade and comparing the results with and without ultrasonic vibration. From experimental results in our group, the use of ultrasound can...

13 June 2024 7,461 4 View

What are the points to be considered for growing MCF7 cells and performing the MTT assay ??

I will be assisting my colleague with MCF7 subculture and later performing MTT assay. We will be using DMEM media (with phenol red) along with 10% FBS and 1% penicillin/streptomycin. We will...

12 June 2024 1,104 3 View

Can someone help me getting the published version of a research article?

I needed help to get the published final version of a research article titled: "Alterations in placental long chain polyunsaturated fatty acid metabolism in human intrauterine growth...

11 June 2024 3,120 2 View