What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning climate change?

Do we have any empirical model to model the combined effect of carbonation and chloride attack in concrete concerning time-dependent temperature, relative humidity and concentration of carbon dioxide?

08 February 2021 4,277 1 View

Which database could provide the signal sequence for targeting various cellular regions? ?

I am searching for thee database which could provide me signal sequence specific to the various cell organelle. such as signal sequence for mitochondria, signal sequence for endoplasmic...

04 February 2021 6,650 2 View

Does AMBER or CHARMM force field parameters have coarsed grained side chain parameters?

I need to coarse grain the side chain with a single atom. However, what force field parameters do I have to use. Does AMBER or CHARMM have any parameters and partial charges for coarse-grained...

31 January 2021 7,091 3 View

Where can I read the force field parameters of amino acids and protein ?

I am a beginner in the field of molecular dynamics and biochemistry. I need the parameters such as van der Waal radii, partial charge of each amino acid. How do I read directly the parameters from...

20 January 2021 3,541 3 View

Can anyone clarify what is the difference between 'R' software statistics and SPSS Statistics?

I recently learned few things about 'R' programming language. It is open source / free platform in many aspects. R helps to descriptive statistics, inferential statistics, multivariate...

17 January 2021 1,089 20 View

Does anyone know what is the minimum variables required for standard deviation? and why large datasets is considered as reliable data?

For Descriptive statistics, we usually find out mean, median and mode as well as standard errors and standard deviation. Almost in research articles, I can see mean with standard deviation only....

10 January 2021 4,913 3 View

For mineral / compound characterization studies like FTIR, SEM, XRD, how many replicates should be taken for data analysis?

If we do some spectroscopy technique for only one sample, is it enough for data analysis or we have to do multiple samples from each treatment for getting authenticate results to publish in...

09 January 2021 5,896 2 View

What is the reference for the partial charges values for peptides shown in avagadro?

I built short peptides in Avagadro, using Build--->insert--->peptide. After constructing the short peptides of 4 amino acids(tetrapeptide). Under the drop-down, View--->Atomic...

27 December 2020 778 3 View

No title

30 November 2020 5,089 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

Is there any method that we can electrochemically oxidize a powder?

I am working with a material which is sensible to water and oxygen, I would like to oxidize this powder either chemical or electromagnetically. Does anyone knows a method to electrochemically...

09 February 2021 2,944 3 View

Please how can i write a proposal on the methodolgies of AAS to determine heavy metalsin water?

Please I want to write a proposal on the methodologies of AAS to determine heavy metals in water. please any link can help.

08 February 2021 3,587 7 View