When should I change the water including antibiotics cocktail for antibiotics-induced microbiota depleted mice (AIMD mice)?
Hello, fella researchers.
I am trying to make AIMD mice for my study.
I have seen many papers to find method.
The antibiotics cocktail including ampicillin (1 g/L), neomycin sulfate (1 g/L), metronidazole (1 g/L) and vancomycin (500 mg/L) will be dissolved into the sterile drink water and provided to mice for 4 weeks.
But, I can't find when should it be changed. Every once in two days? I can't find.
So, If there is anyone who knows this or did this before, please help me.
Hi Carl
See Table 3
https://watermark.silverchair.com/ilv010.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAawwggGoBgkqhkiG9w0BBwagggGZMIIBlQIBADCCAY4GCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQMIzuQktkaJrEYUJIWAgEQgIIBX0_1c9IBK2DhaFHlog-V5DeJjkrm7t8mA89xBwVsZQ-Wgq-4Dp6CdMz_jAUGIGtdnvLlLasnk8BnFQHZFkNVIQHPjWbg-zBFOR_Qr_fLfW48Ava_y54OmEmcKBRbr-19hyOMo1_nKjPgX3iVfm_FLOSkFZ2yRyE3-yOJPhOxzaF3hVcteQGTkEvNQaI82gDkXpc6E8nXk8Ia9uVEN3j3-yeUiWXFrVan92g9YCRLygaEylWh3dRv4pqIic68KsT3IJ9stVXqfqqi6VC68AJxQNqYrjlrA9F45vFWLE0v1YqdqDFsAoARnj8N2oWxCG6WOHypNPIKHb8EvKKOia3XRYUij9Tfd9JDRAsDPstneEsr43VZcHCbPXt9moSS6UL1VYXyCIVe28KikcuorpnL91W_6eYu_fyUYdfgMCGmt_7XtWLcKOdH0XjkUKDGYpi2dPvOQilSzLY5AA5wAX7R0g
Hello, Grant Gallagher.
Thank you for your effort to help. I'm afraid that your link has already been timed out.
If it does not bother you, please post the link again or give me the title of the article.
We have only conducted a similar experiment once, so I cannot speak from much experience, but our lab changed the water twice a week (Mondays and Thursdays, 300mL each time) and that seemed to work well. We also added a little aspartame to offset the bitterness of the antibiotics and verified that the mice were collectively drinking enough by comparing to controls.
Thank you for your information Riccay.
when you started that experiment, did you read any related articles or papers?
Hello Carl,
I do not have a definitive answer to your question, but you might be interested in the following paper. In my experience, metronidazole gives such a foul taste to the drinking water that mice refrain from drinking, losing 20% of their initial weight in less than a week. However, as multiple groups still use this technique, it’s not always the case, and as mentioned by Riccay Elizondo, adding aspartame might help. If your mice experience important weight loss during your protocol, I suggest you consider administering the antibiotics via gavage.
PMID: 21445311
D.H. Reikvam, A. Erofeev, A. Sandvik, V. Grcic, F.L. Jahnsen, P. Gaustad, K.D. McCoy, A.J. Macpherson, L.A. Meza-Zepeda, F.E. Johansen, Depletion of murine intestinal microbiota: Effects on gut mucosa and epithelial gene expression, PLoS One. 6 (2011) 1–13. doi:10.1371/journal.pone.0017996.
Hi Carl. I first use the antibiotic cocktail:
• provided ampicillin (A; 1 g/L; Sigma), vancomycin (V; 500 mg/L; Abbott Labs), neomycin sulfate (N; 1 g/L; Pharmacia/ Upjohn), and metronidazole (M; 1 g/L; Sidmack Labs) in drinking water for four weeks（Wang et al,2015）
However, the ampicillin has strange smelling and mice unwilling to drink it. I then only use remaining 3 antibiotic but the mice still don't want to drink and dehydrated in 5 days. Maybe gavage is a more efficient way.
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