What's the role of mono-Q column in purifying soluble trimerizedinfluenza hemagglutinin protein?

04 April 2015 0 4K Report

Dears,

Again, most papers demonstrated that after the primary purification of HA, then it was further purified with mono-Q column with FPLC.

What's its role in purifying HA? For a better purifty? If so, what's the mechanism in this case even though I know it's an ion-exchange column.

Thanks again,

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