What's the reason for which only monomer protein get detected after the purification of rhBMP-2using CRISPR-casmediated knock-in in Hot spot & HEK293?

23 May 2019 0 6K Report

1. What's the reason for which only monomer protein get detected after the production and purification of rhBMP-2 using CRISPR-cas mediated knock-in in Hot spot and HEK EBNA1(293c18 cell)?

2. What's the considerations for getting homodimer Protein(rhBMP-2) ?

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