Do you want to measure drop in glucose levels in pancreatic cells? If so, you can start with the RIN cell line . Its a pancreatic tumor cell line which secretes high levels of Insulin. You can add glucose to the cell medium and measure its loss over time. But after that, you will have to show this effect in islet cells isolated from mouse/rat pancreas. There are other ways of measuring antidiabetic effect-your question needs more information on what exactly you want to find.
You can use DNSA method. As the result of reaction with reducing sugars (hydrolysis of starch), dinitrosalicylic acid is reduced to nitro aminosalicylic acid (brown).Using spectrophotometer you can measure the absorbance and compare with control. The magnitude of the absorbance is directly proportional to the enzyme activity. The smaller the absorbance, the stronger the inhibition potential of your sample.
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