what's the normal back pressure for Agilent UPLC? When the column is bypassed, the pressure is 50 Bar, but when the column is passed, the pressure reached to 1000 bar..is the tubing clogged?
Dear Min,
Your question in present form can not be answered. The back pressure depends on mobile phase composition, flow rate, column temperature, particle size and column id and length. You didn't give any information.
In addition to the information above, the column type is important too.
To support Dr. Kosyakov's response, I find that on C18, the maximum back pressure is reached between ~55% and 70% organic solvent in water. Furthermore, water/THF solvent systems have higher back pressure than water/methanol.
To check for a clog in the tubing, "follow the flow". Is solvent coming from the pump? Next, check that solvent is coming from the injector. Also watch the pressure as the items are plumbed in. Except for the column, if a large pressure increase is seen when adding a component, that component contains the tubing clog. If you have another column known to be good, try running that (no sample injected) and note the back pressure.
Obviously when you pass through column back pressure will always increase
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