Starch only dissolves in water.  This is the mobile phase (at 85 Celsius) that is used by Waters Sugarpak column (or equivalent).

I don't know if it's compatible with your column, but my colleagues often use DMSO for dissolving starch in their light-scattering experiments.

It's possible, but starch is not as soluble in DMSO (which is polar) as water.

Thanks for replying. I dissolved my starch samples in alkaline solution (1:10 in 6 M Urea and 1M KOH) and neutralized the solution with HCl before I injected the sample in the HPLC. I also tried pure distillied H2O for dissolving my starch sample.

But in both cases I just find an amylopectin peak in my chromatogramm (I am using a POLYSEP-GFC-P linear column for seperating amylose and amylopectin in my strach sample). However, the amylose peak is missing....so I believe it relates to the dissolving cabability of my starch sample?

So, do you think tha DMSO doesnt influenece my POLY-SEP column?Because polymer structure are sensitive to oragnic solvents and could be influenece m the column performance....

It's possible.  All starches are polar or need water in their environment. Even a Waters Sugarpak column can only detect DP6+ (degree of polymerization)

I know, the separation is possible. But I am nor sure that DMSO is a suitable solvent for starch if I use polymer column. polymer material is swelling by organic solvents like methanol....thats why I am not sure if DMSO a proper solvent